Light sheet fluorescence microscopy - Wikipedia
Light sheet fluorescence microscopy (LSFM) is a fluorescence microscopy technique with an intermediate-to-high [1] optical resolution, but good optical sectioning capabilities and high speed. In contrast to epifluorescence microscopy only a thin slice (usually a few hundred nanometers to a few micrometers) of the sample is illuminated ...
前沿显微成像技术专题之：光片显微镜（2） - 知乎专栏
2019年4月11日 · 前沿显微成像技术专题又和大家见面啦！上一期我们简单介绍了光片荧光显微镜的一些基本知识，光片显微镜的诞生大大拓展了生命科学的研究视野，但它也有一些需要克服的天生缺陷和技术难点。本期就让我们从这里开始，…
Light sheet fluorescence microscopy | Nature Reviews Methods …
2021年11月3日 · Light sheet fluorescence microscopy (LSFM) is a technique that uses a thin sheet of light for illumination, allowing optical sectioning of the sample.
What Is Light Sheet Microscopy | Teledyne Vision Solutions
Light Sheet Microscopy. In typical microscopy techniques, the illumination and imaging systems use the same light path and are on the same axis. In light sheet microscopy, the illumination beam is perpendicular to the imaging system (Figure 1D, Figure 2), creating a sheet of light through a particular 2D plane of the sample.
蔡司Lightsheet 7——光片显微镜 - ZEISS Vision Care
光片荧光显微技术（LSFM）非常适合对整个活体模式生物、组织或细胞在很长一段时间内的发育过程进行快速而低光毒性的成像。 另外，使用蔡司Lightsheet 7还能够以亚细胞分辨率对大尺寸透明化样品进行完整成像。 专用的元件、样品仓和样品载具可适应您所选择的透明化方法的折射率。 高量子效率的探测器能够在很低的照明水平下观察到快速变化的过程。 您将获得样品的真实生命视图，而激发光不会对其生理造成负面影响。 特殊样品仓可提供加热、冷却及二氧化碳（CO …
Light Sheet Fluorescence Microscopy | Nikon’s MicroscopyU
Light Sheet Fluorescence Microscopy (LSFM) is a general name for a constantly growing family of planar illumination techniques that have revolutionized how optical imaging of biological specimens can be performed. Fundamentally, LSFM techniques are made possible by decoupling the illumination and detection optical pathways, allowing for novel ...
A guide to light-sheet fluorescence microscopy for multiscale imaging
2017年3月31日 · This Review introduces the fundamental considerations for building a light sheet microscope, describes the pros and cons associated with available implementations, and offers practical advice for...
Light Sheet Fluorescence Microscopy | 爱特蒙特光学 - Edmund …
Light sheet fluorescence microscopy (LSFM, also known as selective plane illumination microscopy (SPIM), is a medium-to-high-resolution fluorescence microscopy method that captures images at high speeds. It is well-suited for imaging three-dimensional biological samples at multiple depths, known as optical sectioning.
Light-sheet microscopy | Hamamatsu Photonics
A light-sheet microscope is an observation technique that illuminates a sample with sheet-shaped light to acquire 2D images. By changing the position where the sheet light is applied, it also enables 3D imaging.
What is Light-Sheet Microscopy - Bruker
Light-sheet microscopy is a fluorescence imaging technique, which utilizes a sheet of laser light to illuminate only a thin slice of the sample. The basic technical principle is a wide-field fluorescence microscope, placed perpendicular to the light-sheet, that collects the fluorescence signal and images of the observed region by means of a ...
- 某些结果已被删除