Imaging with better than 200-nanometer resolution brings new subcellular-scale details into focus. Practitioners share how they weigh trade-offs in speed, resolution and phototoxicity.

Conventional light microscopes have a resolution limit of 200 nanometers, meaning that any objects closer than this distance will not be observed as separate objects.

Super-resolution microscopy (SRM) encompasses multiple techniques that achieve higher resolution than traditional light microscopy. The resolution of conventional light microscopy is limited to around ...

The intricate, hidden processes that sustain coral life are being revealed through a new microscope developed by scientists ...

Conventional light microscopes typically have a resolution limit of 200nm, meaning that any objects closer than this distance will not be observed as separate objects. While there are more powerful ...

Posted: Aug 13, 2007: Nanoscopy - nanoscale resolution in light microscopy (Nanowerk Spotlight) In the early 1870s, the German physicist Ernst Karl Abbé formulated a rigorous criterion for being able ...

Light-shrinking material lets ordinary microscope see in super resolution. ScienceDaily . Retrieved May 9, 2025 from www.sciencedaily.com / releases / 2021 / 06 / 210601100729.htm ...

These studies demonstrate for the first time that resolution below a half-wavelength of visible light is no longer reserved for the electron microscope when observing cells. As demonstrated by ...

Here, researchers have access to many imaging systems, including transmitted light, TIRF, and epifluorescence microscopes, spinning disc and laser scanning confocal microscopes, 2-photon systems, and ...

Beyond adapting the optical system to a specific use case, modern light microscopy also takes advantage of digital image processing, such as image deconvolution. 12 This technique allows the increase ...

“Our strategy for dense light-microscopy-based connectomics achieves an increase in resolution through hydrogel expansion rather than optical super-resolution,” they further explained.