After one additional freeze-thaw cycle, a brief centrifugation (500 g, 5 minutes, 10 °C) was made to clear the lysate from cell debris. Then the viruses were pelleted (10.000 g, 60 minutes, 10 °C ...
A Complete Unknown' stars — and director James Mangold — on channeling Bob Dylan, surviving sudden fame and the pitfalls of working with your rock idols.
More than 60 Catholic Benedictine monks reside here ... but also the site of Milan High School's 1954 championship title that inspired the story in "Hoosiers," starring Gene Hackman. The movie itself ...