研究人员针对神经发育过程中细胞信号传导与分子状态关联的测量难题，开发了名为Phospho-seq的多模态单细胞技术。该研究通过优化抗体偶联方法，实现了对64种细胞内蛋白（含20种磷酸化状态）与染色质可及性（scATAC-seq）的同步检测，并结合实验与计算策略整合转录组数据。

中国科学院上海药物研究所研究员谭敏佳课题组和江苏海洋大学教授刘彬团队合作，在Cell Death & Differentiation上，在线发表了题为Global identification of ...

Specific arrestin conformations are coupled to distinct downstream effectors, which underlie the functions of many G-protein-coupled receptors (GPCRs). Here, using unnatural amino acid ...

The role of protein phosphorylation in the life cycle of malaria parasites is slowly emerging. Here we combine global phospho-proteomic analysis with kinome-wide reverse genetics to assess the ...

Figure 1. Main readouts for GPCR signaling pathway investigations, including cyclic AMP, IP1, and p-ERK. Advanced Phospho-ERK1/2 Assay. These assay miniaturization and detection issues have led ...

Proteome and phospho-proteome profiling for deeper phenotype characterization of colorectal cancer heterogeneity. Authors: Jakob Vowinckel, Thomas Corwin, Jonathan Woodsmith, Tobias Treiber, Roland ...

Development of microscopy-based assays to quantify levels of phospho-MET (pMET) in tumors has been hampered by poor antibody specificity. Here, we present the development and validation of a robust, ...

Fixation Before Dissociation Using a Deep Eutectic Solvent Preserves In Vivo States and Phospho-Signalling in Single-Cell Sequencing Presented. News-Medical. Retrieved on June 19, ...

They saw no differences at several tau sites typically phosphorylated in 3xTg-AD mice by eight months (Thr212/Ser214, Ser199/202, or Thr181), but a whopping 60 percent reduction in Thr231-phospho-tau ...

Mass spectrometry picked up the lowest concentration of phospho-tau ever detected in plasma. P-tau-217 picked out people with brain amyloid even better than p-tau-181. A plasma test for Aβ42 highly ...