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生物通
5 年
PLoS Biology:新的小型CRISPR/Cas9工具 - 生物通
该Cas9来源于耳氏葡萄球菌(Staphylococcus auricularis),被命名为SauriCas9,识别NNGG PAM序列,编辑范围与常用的SpCas9一样。 SauriCas9通过AAV病毒包装后,在多种细胞类型中实现了基因编辑(图),说明SauriCas9具有用于基因治疗的潜力。
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